8CLY

Crystal structure of Rhizobium etli constitutive L-asparaginase ReAIV (tetragonal form R4tP)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-23
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9700
Spacegroup name	P 42 21 2
Unit cell lengths	125.344, 125.344, 115.227
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	88.630 - 2.503
Rwork	0.172
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.517
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	88.630	2.650
High resolution limit [Å]	2.500	2.500
Rmerge	0.176	1.016
Rmeas	0.187	1.079
Number of reflections	32235	5074
<I/σ(I)>	11.41	2.16
Completeness [%]	99.9
Redundancy	8.82
CC(1/2)	0.996	0.739

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium formate, 20 % (w/v) PEG 3350, and 0.2% lauryldimethylamine oxide (LDAO)