8CLV
Zearalenone lactonase of Rhodococcus erythropolis in complex with hydrolyzed zearalenone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-05 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 123.380, 172.090, 136.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.050 - 2.540 |
| R-factor | 0.2154 |
| Rwork | 0.214 |
| R-free | 0.24610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.582 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4489) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.050 | 2.631 |
| High resolution limit [Å] | 2.540 | 2.540 |
| Rmerge | 0.220 | 1.984 |
| Rmeas | 0.231 | 2.096 |
| Rpim | 0.069 | 0.652 |
| Number of reflections | 44439 | 4434 |
| <I/σ(I)> | 9.63 | 1.37 |
| Completeness [%] | 92.3 | 92.95 |
| Redundancy | 10.5 | 9.8 |
| CC(1/2) | 0.995 | 0.221 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295.15 | 0.2 M NaCl, 0.1 M BIS-TRIS 5.5 pH, 25% w/v PEG 3350 |
