8CLQ
Zearalenone lactonase of Streptomyces coelicoflavus mutant H286Y in complex with hydrolyzed zearalenone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.809, 129.867, 134.822 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.770 - 1.530 |
| R-factor | 0.1978 |
| Rwork | 0.198 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.736 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4489) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.770 | 1.585 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Rmerge | 0.204 | 4.244 |
| Rmeas | 0.222 | 4.664 |
| Rpim | 0.086 | 1.902 |
| Number of reflections | 174008 | 17042 |
| <I/σ(I)> | 7.01 | 0.37 |
| Completeness [%] | 98.3 | 85.93 |
| Redundancy | 6.6 | 5.8 |
| CC(1/2) | 0.997 | 0.071 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295.15 | 0.1 M Mg formate dihydrate 10.09% PEG 3350 (pH 6.9) |
