8CLO

Zearalenone lactonase from Streptomyces coelicoflavus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.8726
Spacegroup name	C 1 2 1
Unit cell lengths	173.548, 69.297, 64.423
Unit cell angles	90.00, 96.06, 90.00

Refinement procedure

Resolution	46.310 - 1.400
R-factor	0.1871
Rwork	0.187
R-free	0.21600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.014
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	PHENIX (1.20.1-4489)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.310	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.120	1.534
Rmeas	0.137	1.801
Rpim	0.065	0.922
Number of reflections	148595	14556
<I/σ(I)>	6.18	0.86
Completeness [%]	99.5	97.86
Redundancy	4.2	3.5
CC(1/2)	0.993	0.298

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295.15	0.1 M Imidazole / MES pH 6.5, 0.06 M MgCl2, CaCl2, 30% w/v {40% ethylene glycol, 20% PEG 8000}