8CL8

Krokinobacter eikastus rhodopsin 2 (KR2) extrapolated map 1us after light activation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SwissFEL ARAMIS BEAMLINE ESA
Synchrotron site	SwissFEL ARAMIS
Beamline	ESA
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2021-09-16
Detector	PSI JUNGFRAU 16M
Wavelength(s)	1.028919
Spacegroup name	I 2 2 2
Unit cell lengths	41.460, 84.890, 234.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.830 - 2.400
R-factor	0.3284
Rwork	0.327
R-free	0.35830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.925
Data reduction software	CrystFEL (0.9.1)
Data scaling software	CrystFEL (0.9.1)
Phasing software	PHASER (1.20_4459)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.100	1.830
High resolution limit [Å]	1.760	1.760
Number of reflections	26536	172
<I/σ(I)>	6.18	1.23
Completeness [%]	60.4	3.98
Redundancy	400.15
CC(1/2)	0.966	0.315

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293.15	200 mM Sodium acetate pH 4.4 150 mM Magnesium Chloride 34 % PEG 200