8CL7

Krokinobacter eikastus rhodopsin 2 (KR2) in dark state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SwissFEL ARAMIS BEAMLINE ESA
Synchrotron site	SwissFEL ARAMIS
Beamline	ESA
Temperature [K]	293.15
Detector technology	PIXEL
Collection date	2021-09-16
Detector	PSI JUNGFRAU 16M
Wavelength(s)	1.028919
Spacegroup name	I 2 2 2
Unit cell lengths	41.460, 84.890, 234.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.970 - 1.760
R-factor	0.1573
Rwork	0.155
R-free	0.18450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.166
Data reduction software	CrystFEL (0.9.1)
Data scaling software	CrystFEL (0.9.1)
Phasing software	PHASER (1.20_4459)
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.976	1.830
High resolution limit [Å]	1.760	1.760
Number of reflections	28894	370
<I/σ(I)>	8.17	1.46
Completeness [%]	65.7
Redundancy	960.89	234.7
CC(1/2)	0.986	0.483

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293.15	200 mM Sodium acetate pH 4.4 150 mM Magnesium Chloride 34 % PEG 200