8CKM

Semaphorin-5A TSR 3-4 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	C 1 2 1
Unit cell lengths	104.110, 27.650, 92.240
Unit cell angles	90.00, 99.10, 90.00

Refinement procedure

Resolution	51.400 - 2.720
R-factor	0.29
Rwork	0.288
R-free	0.33110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.506
Data reduction software	XDS (20200417)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.19rc5_4047)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.410	2.770
High resolution limit [Å]	2.720	2.720
Rmerge	0.114	5.129
Rmeas	0.136	6.373
Rpim	0.073	3.725
Number of reflections	7316	382
<I/σ(I)>	7.1	0.3
Completeness [%]	99.8
Redundancy	6.1
CC(1/2)	0.994	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	38 % PEG 200, 0.1 M sodium citrate