8CK6
Crystal structure of maize CKO/CKX8 in complex with urea-derived inhibitor 2-[(3,5-dichlorophenyl)carbamoylamino]-4-methoxy-benzamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 65 |
| Unit cell lengths | 101.700, 101.700, 128.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.040 - 1.900 |
| R-factor | 0.175 |
| Rwork | 0.174 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS (BUILT=20220220) |
| Data scaling software | XDS (BUILT=20220220) |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.300 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.210 | 2.228 |
| Rmeas | 0.216 | 2.211 |
| Rpim | 0.046 | 0.472 |
| Number of reflections | 59038 | 9517 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.3 | 21.3 |
| CC(1/2) | 0.998 | 0.668 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 15% PEG 1500, 50 mM Tris-HCl pH 8.0, 1.5 mM inhibitor, 20% glycerol for cryoprotection |
