8CJ8
Arabidopsis thaliana Phosphoenolpyruvate carboxylase PPC1 mutant A651V in complex with L-malate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.981 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 245.380, 245.380, 397.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.716 - 3.490 |
| R-factor | 0.245546637964 |
| Rwork | 0.244 |
| R-free | 0.27394 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.086 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.716 | 3.700 |
| High resolution limit [Å] | 3.490 | 3.490 |
| Rmerge | 0.450 | 2.716 |
| Number of reflections | 76655 | 11923 |
| <I/σ(I)> | 5.32 | |
| Completeness [%] | 99.5 | 79.5 |
| Redundancy | 26.42 | 24.7 |
| CC(1/2) | 0.997 | 0.791 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 30% w/v PEG400; 0,1M MES pH6,5; 0,1M NaAc |
