8CJ5
Arabidopsis thaliana Phosphoenolpyruvate carboxylase PPC1 A651V mutant with bound phosphate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.981 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 128.830, 156.620, 142.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.039 - 3.001 |
R-factor | 0.217167515667 |
Rwork | 0.215 |
R-free | 0.26578 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.849 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.040 | 3.500 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.242 | 3.867 |
Number of reflections | 58634 | 8677 |
<I/σ(I)> | 8.6 | 0.39 |
Completeness [%] | 99.6 | |
Redundancy | 10.34 | 8.7 |
CC(1/2) | 0.996 | 0.838 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 293 | 0.09M Sodium nitrate, 0.09 Sodium phosphate dibasic, 0.09M Ammonium sulfate, 0.1M MES monohydrate pH 6.5, 20% v/v Ethylene glycol; 10 % w/v PEG 8K |