8CIY

DNA-polymerase sliding clamp (DnaN) from Escherichia coli in complex with Cyclohexyl-Griselimycin.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000000
Spacegroup name	C 2 2 2
Unit cell lengths	83.907, 150.723, 71.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 1.540
R-factor	0.1724
Rwork	0.171
R-free	0.20010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.996
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.980	1.620
High resolution limit [Å]	1.540	1.540
Rmerge	0.061	0.918
Rmeas	0.063	0.957
Rpim	0.017	0.268
Total number of observations	901633	123025
Number of reflections	67324	9751
<I/σ(I)>	21.4	2.6
Completeness [%]	100.0
Redundancy	13.4	12.6
CC(1/2)	0.999	0.876

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	293	0.64 M CaCl2 0.5 M Li Acetate 9.4 % (w/v) PEG 8000 0.1 M HEPES/NaOH 7.7 Cryoprotection: 10 % (v/v) (2R,3R)-2,3-butanediol