8CIX

DNA-polymerase sliding clamp (DnaN) from Escherichia coli in complex with Griselimycin.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	C 2 2 2
Unit cell lengths	83.936, 150.561, 71.594
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.880 - 1.760
R-factor	0.1801
Rwork	0.179
R-free	0.20530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.035
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.280	1.860
High resolution limit [Å]	1.760	1.760
Rmerge	0.084	1.030
Rmeas	0.088	1.069
Rpim	0.024	0.288
Total number of observations	604946	89664
Number of reflections	45185	6526
<I/σ(I)>	17.9	2.5
Completeness [%]	100.0
Redundancy	13.4	13.7
CC(1/2)	1.000	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.7	293	0.36 M CaCl2 0.13 M MgCl2 0.24 M Na Acetate 8.3 % (v/v) Glycerol 12.2 % (w/v) PEG 3350 0.1 M HEPES/NaOH 7.7 Cryoprotection: 10 % (v/v) (2R,3R)-2,3-butanediol.