8CIT
The FERM domain of human moesin mutant L281R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-01-20 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.704, 74.049, 115.075 |
| Unit cell angles | 90.00, 95.63, 90.00 |
Refinement procedure
| Resolution | 62.180 - 2.536 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.28750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.030 |
| Data reduction software | DIALS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.200 | 3.070 |
| High resolution limit [Å] | 2.536 | 2.540 |
| Number of reflections | 23727 | 3949 |
| <I/σ(I)> | 4.3 | 1.5 |
| Completeness [%] | 58.5 | |
| Redundancy | 7 | |
| CC(1/2) | 0.976 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 200 mM sodium fluoride, 100 mM bris-tris-propane, 10% ethylene glycol, 20% PEG3350 |
