8CIG
Crystal structure of an 8-repeat consensus TPR superhelix in tris Buffer with Calcium.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 68.046, 68.046, 72.285 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.930 - 1.400 |
| R-factor | 0.16966 |
| Rwork | 0.169 |
| R-free | 0.19169 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.750 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP (11.7.02) |
| Refinement software | REFMAC (5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.280 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmeas | 0.148 | 2.270 |
| Rpim | 0.035 | 0.535 |
| Total number of observations | 675371 | 98744 |
| Number of reflections | 38606 | 5569 |
| <I/σ(I)> | 11.3 | 2.2 |
| Completeness [%] | 100.0 | |
| Redundancy | 17.5 | 17.7 |
| CC(1/2) | 0.996 | 0.689 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 292 | 100 mM Tris-HCl, pH 8.0, 20 mM CaCl2, 30% MPD |
