8CIF
Bovine naive ultralong antibody AbD08 collected at 293K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE VMXi |
| Synchrotron site | DIAMOND |
| Beamline | VMXi |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2019-12-02 |
| Detector | DECTRIS EIGER2 X 4M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.228, 71.585, 100.233 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.320 - 2.200 |
| R-factor | 0.17963 |
| Rwork | 0.177 |
| R-free | 0.22786 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.522 |
| Data reduction software | DIALS (3.6.0-2-g5baae39f5-release) |
| Data scaling software | DIALS (3.6.0-2-g5baae39f5-release) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.470 | 2.110 |
| High resolution limit [Å] | 2.430 | 2.430 | 2.070 |
| Rmerge | 0.197 | 1.227 | 0.831 |
| Number of reflections | 14787 | 758 | 619 |
| <I/σ(I)> | 3.5 | 0.4 | 0.5 |
| Completeness [%] | 82.4 | ||
| Redundancy | 2.2 | ||
| CC(1/2) | 1.000 | 0.300 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris (base) 0.1M Bicine 12% (v/v) PEG 500 MME 6% (v/v) PEG 20000 0.15M Sodium chloride 0.09M Sodium fluoride 0.09M Sodium iodide 0.09M Sodium bromide |
| 2 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris (base) 0.1M Bicine 12% (v/v) PEG 500 MME 6% (v/v) PEG 20000 0.15M Sodium chloride 0.09M Sodium nitrate 0.09M Sodium phosphate dibasic 0.09M Ammonium sulphate |
| 3 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris (base) 0.1M Bicine 12% (v/v) PEG 500 MME 6% (v/v) PEG 20000 0.15M Sodium chloride 0.09M Sodium nitrate 0.09M Sodium phosphate dibasic 0.09M Ammonium sulphate |
| 4 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1M Tris (base) 0.1M Bicine 12% (v/v) PEG 500 MME 6% (v/v) PEG 20000 0.15M Sodium chloride 0.1M Sodium formate 0.1M Ammonium Acetate 0.1M Sodium citrate tribasic dihydrate 0.1M Potassium sodium tartrate tetrahydrate 0.1M Sodium oxamate |
