8CID
Crystal structure of Oryza sativa UAM 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97741 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 115.390, 115.390, 157.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.160 - 3.600 |
| R-factor | 0.2469 |
| Rwork | 0.244 |
| R-free | 0.27250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.965 |
| Data reduction software | MOSFLM |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.200 | 3.800 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmeas | 0.197 | 0.831 |
| Number of reflections | 12771 | 1256 |
| <I/σ(I)> | 5.3 | 1.7 |
| Completeness [%] | 98.9 | 99.6 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 100 mM HEPES pH 7.5, 25%(v/v) PEG 3350, 200 mM MgCl2 |
