8CI0
Maize Transketolase in complex with TPP and hydrolyzed (+)-Cornexistin
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION NOVA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-10-07 |
| Detector | OXFORD ONYX CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 135.309, 135.309, 201.439 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.727 - 1.902 |
| Rwork | 0.153 |
| R-free | 0.18360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.544 |
| Data reduction software | CrysalisPro |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.830 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.175 |
| Number of reflections | 157602 | 18870 |
| <I/σ(I)> | 19 | |
| Completeness [%] | 94.4 | |
| Redundancy | 6.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 28 % PEG 3350, 250 mM ammonium acetate |
