8CHW
Crystal structure of human PURA (fragment Pro216-Lys280, PUR repeat III)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-01 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.000040 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 56.760, 75.640, 31.390 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.850 - 1.700 |
| R-factor | 0.1783 |
| Rwork | 0.176 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.569 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 15494 | 1142 |
| <I/σ(I)> | 19.5 | 6.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.4 | |
| CC(1/2) | 1.000 | 0.887 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 0.1 M Tris pH 8.5, 12% (v/v) glycerol, 1.5 M (NH4)2SO4 |
