8CHD

NtUGT1 in two conformations

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	52.310, 86.850, 100.980
Unit cell angles	90.00, 93.17, 90.00

Refinement procedure

Resolution	50.410 - 2.340
R-factor	0.1912
Rwork	0.189
R-free	0.23450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.726
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.410	2.420
High resolution limit [Å]	2.340	2.340
Number of reflections	37833	3374
<I/σ(I)>	10.02	1.23
Completeness [%]	98.6	88.34
Redundancy	3.7	3.2
CC(1/2)	0.995	0.430

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2 M magnesium chloride 25% PEG 3350 0.1 M HEPES