8CHA

Fc gamma RIIa 27W/131H variant ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-07
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	49.931, 76.526, 108.279
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.817 - 1.810
R-factor	0.1944
Rwork	0.193
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ry4
RMSD bond length	0.004
RMSD bond angle	0.756
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.13)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.820	1.875
High resolution limit [Å]	1.810	1.810
Rmerge	0.150	0.730
Rmeas	0.160	0.830
Rpim	0.060	0.390
Number of reflections	19293	1867
<I/σ(I)>	6.3	1.4
Completeness [%]	99.0	96.8
Redundancy	6.1
CC(1/2)	0.990	0.450

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293.15	0.1M sodium phosphate citrate, pH4.2, 41% (v/v) PEG 300