8CGX

structure of HEX-1 from N. crassa crystallized in cellulo, diffracted at 100K and resolved using XDS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.980
Spacegroup name	P 65 2 2
Unit cell lengths	57.240, 57.240, 198.190
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.030 - 1.850
R-factor	0.206
Rwork	0.204
R-free	0.23320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.865
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.030	1.916
High resolution limit [Å]	1.850	1.850
Number of reflections	33344	1639
<I/σ(I)>	35.15
Completeness [%]	99.8
Redundancy	4639
CC(1/2)	0.999	0.939

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		300	baculovirus infected and grown in Trichoplusia ni (High Five) cells in adhesion culture (MOI 1)