8CFF
Crystal structure of arsenite oxidase from Alcaligenes faecalis (Af Aio) bound to arsenite
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.306, 108.982, 116.892 |
| Unit cell angles | 97.50, 90.21, 96.06 |
Refinement procedure
| Resolution | 65.620 - 1.570 |
| R-factor | 0.16974 |
| Rwork | 0.168 |
| R-free | 0.20439 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.886 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.620 | 1.690 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Number of reflections | 452928 | 22648 |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 74.5 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.989 | 0.780 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 10% v/v PEG 4000, 0.1 M sodium citrate pH 5.5 and 10% v/v isopropanol |
