8CE9

Crystal structure of human Cd11b I domain in C121 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-29
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	C 1 2 1
Unit cell lengths	128.495, 51.494, 90.172
Unit cell angles	90.00, 132.96, 90.00

Refinement procedure

Resolution	65.989 - 2.110
Rwork	0.188
R-free	0.23890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.609
Data reduction software	xia2
Data scaling software	xia2
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.990	2.150
High resolution limit [Å]	2.110	2.110
Number of reflections	23208	728
<I/σ(I)>	7.5
Completeness [%]	92.5
Redundancy	6.4
CC(1/2)	0.981	0.298

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	25% PEG Smear Medium 0.1M cacodylate pH 5.5 0.2M ammonium sulfate