8CDF
Structure of glutarate hydroxylase (GlaH) from Escherichia coli at a resolution of 1.8 angstrom obtained as a contaminant during routine use of E. coli as an expression host
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 121.132, 121.132, 136.487 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.350 - 1.770 |
| R-factor | 0.2017 |
| Rwork | 0.201 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.879 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.370 | 1.800 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.102 | |
| Rmeas | 0.106 | |
| Number of reflections | 49508 | 2412 |
| <I/σ(I)> | 11.9 | 0.3 |
| Completeness [%] | 100.0 | 99.1 |
| Redundancy | 11.9 | 6 |
| CC(1/2) | 0.999 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291.15 | 47 mM K3PO4, 0.4 M (NH4)2SO4, 200 mM KCl, 1mM EDTA |
