8CD6

structure of HEX-1 (cyto V2) from N. crassa grown in living insect cells, diffracted at 100K and resolved using CrystFEL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.976
Spacegroup name	P 65 2 2
Unit cell lengths	56.700, 56.700, 196.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.100 - 1.850
R-factor	0.2035
Rwork	0.201
R-free	0.23670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1khi
RMSD bond length	0.008
RMSD bond angle	0.904
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	1.916
High resolution limit [Å]	1.850	1.850
Number of reflections	30212	1646
<I/σ(I)>	8.15
Completeness [%]	99.8
Redundancy	278
CC(1/2)	0.995	0.423

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		300	baculovirus infected and grown in Trichoplusia ni (High Five) cells in adhesion culture (MOI 1)