8CBP
The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with tetrapeptide Thr-Glu-Pro-Leu
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.194, 81.390, 60.630 |
Unit cell angles | 90.00, 112.28, 90.00 |
Refinement procedure
Resolution | 48.360 - 2.700 |
R-factor | 0.245 |
Rwork | 0.242 |
R-free | 0.29610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hck |
RMSD bond length | 0.002 |
RMSD bond angle | 0.499 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.400 | 2.830 |
High resolution limit [Å] | 2.700 | 2.700 |
Rpim | 0.047 | 0.782 |
Number of reflections | 13994 | 1389 |
<I/σ(I)> | 10.5 | 1.2 |
Completeness [%] | 99.7 | 97.6 |
Redundancy | 3.9 | |
CC(1/2) | 0.997 | 0.767 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | Protein: 3.1-6.6 mg mL-1, 20 mM NaP pH 6.5, 200 mM NaCl was mixed with peptides (0.5-2.5% DMSO, 1-4 mM DTT) in ratio 1:10. Well solution: 100 mM Nacitrate pH 5.1-5.7, 16-26% PEG4000. Dropsize 1:1 microL Cryoprotection: 35% PEG4000 |