8CBI

The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with tetrapeptide Thr-Lys-Pro-Arg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-26
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	56.403, 80.953, 61.793
Unit cell angles	90.00, 110.74, 90.00

Refinement procedure

Resolution	48.420 - 2.400
R-factor	0.2199
Rwork	0.218
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.420	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.122	1.225
Number of reflections	20408	2019
<I/σ(I)>	5.6	1.2
Completeness [%]	100.0	100
Redundancy	4.6	4.7
CC(1/2)	0.989	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	Protein: 3.1-6.6 mg mL-1, 20 mM NaP pH 6.5, 200 mM NaCl was mixed with peptides (0.5-2.5% DMSO, 1-4 mM DTT) in ratio 1:10. Well solution: 100 mM Nacitrate pH 5.1-5.7, 16-26% PEG4000. Dropsize 1:1 microL Cryoprotection: 35% PEG4000