8CB7

The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with tetrapeptide Glu-Val-Phe-Leu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-26
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	48.620, 94.242, 101.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.700 - 2.850
R-factor	0.2636
Rwork	0.259
R-free	0.30660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6hck
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.700	2.950
High resolution limit [Å]	2.850	2.850
Rpim	0.107	1.013
Number of reflections	11417	1113
<I/σ(I)>	7.7	1.2
Completeness [%]	100.0	100
Redundancy	8.6	9.1
CC(1/2)	0.993	0.403

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	Protein: 3.1-6.6 mg mL-1, 20 mM NaP pH 6.5, 200 mM NaCl was mixed with peptides (0.5-2.5% DMSO, 1-4 mM DTT) in ratio 1:10. Well solution: 100 mM Nacitrate pH 5.1-5.7, 16-26% PEG4000. Dropsize 1:1 microL Cryoprotection: 35% PEG4000