8CB5
The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with tripeptide Glu-Val-Phe
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.197, 87.306, 112.642 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.330 - 2.250 |
R-factor | 0.2164 |
Rwork | 0.214 |
R-free | 0.26470 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.330 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rpim | 0.053 | 0.590 |
Number of reflections | 23242 | 2263 |
<I/σ(I)> | 12.9 | 1.3 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.7 | 13.4 |
CC(1/2) | 0.999 | 0.546 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | Protein: 3.1-6.6 mg mL-1, 20 mM NaP pH 6.5, 200 mM NaCl was mixed with peptides (0.5-2.5% DMSO, 1-4 mM DTT) in ratio 1:10. Well solution: 100 mM Nacitrate pH 5.1-5.7, 17% isopropanol, 16-26% PEG4000. Dropsize 1:1 microL Cryoprotection: 35% PEG4000 |