8CAG

Hypoxanthine-guanine phosphoribosyltransferase from E. coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-03
Detector	DECTRIS EIGER R 1M
Wavelength(s)	0.8856
Spacegroup name	P 31 2 1
Unit cell lengths	84.650, 84.650, 167.242
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.740 - 2.400
R-factor	0.25577
Rwork	0.254
R-free	0.29283
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.693
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.235	0.290
Number of reflections	27430	3993
<I/σ(I)>	2
Completeness [%]	98.8
Redundancy	2.97

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	ammonium sulfate