8C88

Double mutant G(M19)C/T(L214)C structure of Photosynthetic Reaction Center From Cereibacter sphaeroides strain RV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-08
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 42 21 2
Unit cell lengths	100.910, 100.910, 237.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.420 - 2.750
R-factor	0.202
Rwork	0.199
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.234
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.820
High resolution limit [Å]	2.750	2.750
Rmeas	0.110	1.240
Number of reflections	32119	2369
<I/σ(I)>	7.92	1.06
Completeness [%]	98.1
Redundancy	3.6
CC(1/2)	0.990	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	1-mono-oleyl-rac-glycerol (MO), 1,2,3-heptanetriol, Jeffamine M600, N,N-dimethyldodecylamine-N-oxide (LDAO)