8C84

Crystal structure of MADS-box/MEF2D N-terminal domain complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-09
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9724
Spacegroup name	P 21 21 21
Unit cell lengths	53.033, 56.364, 75.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.190 - 1.900
R-factor	0.20619
Rwork	0.204
R-free	0.25118
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.882
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0403)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.190	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.070	0.893
Number of reflections	18562	1182
<I/σ(I)>	15.1
Completeness [%]	99.8
Redundancy	8.1
CC(1/2)	0.999	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM sodium acetate trihydrate, 100 mM Bis-Tris propane pH 6.5 and 20% w/v polyethylene glycol 3350 (PEG 3350)