8C7V
Phage display derived serum albumin binding knob domain engineered within a novel VH framework 3 bispecific antibody format
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 196 |
| Detector technology | PIXEL |
| Collection date | 2013-09-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.600, 127.930, 93.810 |
| Unit cell angles | 90.00, 95.47, 90.00 |
Refinement procedure
| Resolution | 60.320 - 1.610 |
| R-factor | 0.1676 |
| Rwork | 0.166 |
| R-free | 0.19430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.064 |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.900 | 1.650 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Rmerge | 0.034 | |
| Number of reflections | 178845 | 29677 |
| <I/σ(I)> | 17 | 2.2 |
| Completeness [%] | 97.5 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.999 | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | PEG 3350 malic acid |
