8C7V

Phage display derived serum albumin binding knob domain engineered within a novel VH framework 3 bispecific antibody format

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	196
Detector technology	PIXEL
Collection date	2013-09-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	60.600, 127.930, 93.810
Unit cell angles	90.00, 95.47, 90.00

Refinement procedure

Resolution	60.320 - 1.610
R-factor	0.1676
Rwork	0.166
R-free	0.19430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.064
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.900	1.650
High resolution limit [Å]	1.610	1.610
Rmerge	0.034
Number of reflections	178845	29677
<I/σ(I)>	17	2.2
Completeness [%]	97.5
Redundancy	3.3
CC(1/2)	0.999	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	PEG 3350 malic acid