8C7J

Phage display derived serum albumin binding knob domain engineered within a novel VH framework 3 bispecific antibody format

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	196
Detector technology	PIXEL
Collection date	2022-04-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	63.240, 71.310, 125.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.940 - 2.000
R-factor	0.188
Rwork	0.186
R-free	0.22590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.095
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.940	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.072	0.441
Rmeas	0.075	0.469
Rpim	0.021	0.155
Number of reflections	39199	3837
<I/σ(I)>	19.71	4.43
Completeness [%]	99.9	99.12
Redundancy	12.6	8.7
CC(1/2)	0.999	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	PEG 3350, lithium chloride