8C7I

Crystal structure of the PS2 assembly factor Psb32 from the cyanobactium Thermosyncechococcus vestitus (formerly elongatus)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-12
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.95107
Spacegroup name	P 61 2 2
Unit cell lengths	145.650, 145.650, 91.810
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.680 - 2.120
R-factor	0.2288
Rwork	0.227
R-free	0.25800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.796
Data reduction software	XDS (VERSION Jan 10, 2022 BUILT=20220120)
Data scaling software	XSCALE (VERSION Jan 10, 2022 BUILT=20220120)
Phasing software	BALBES
Refinement software	PHENIX (dev_4788)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.680	2.180
High resolution limit [Å]	2.120	2.120
Rmerge	0.128	6.660
Rmeas	0.130	6.760
Number of reflections	1158546	88765
<I/σ(I)>	24.87	1.01
Completeness [%]	99.3	98.5
Redundancy	35.3	37.5
CC(1/2)	1.000	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	291	1 M sodium citrate, 0.1 M CHES pH 9.5