8C7D
Structure of the GEF Kalirin DH2 Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.324, 74.478, 100.121 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.760 - 1.860 |
| R-factor | 0.2298 |
| Rwork | 0.227 |
| R-free | 0.27450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.930 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.760 | 1.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmeas | 0.261 | |
| Rpim | 0.076 | |
| Number of reflections | 17994 | 881 |
| <I/σ(I)> | 7.1 | 0.4 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 11.7 | 12.9 |
| CC(1/2) | 0.998 | 0.351 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% W/V PEG3350, 0.1 M succinic acid |
