8C75
PBP AccA from A. tumefaciens Bo542 in apoform 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.990, 182.075, 58.606 |
| Unit cell angles | 90.00, 94.09, 90.00 |
Refinement procedure
| Resolution | 49.190 - 1.673 |
| R-factor | 0.2212 |
| Rwork | 0.219 |
| R-free | 0.25590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.090 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.190 | 1.710 |
| High resolution limit [Å] | 1.660 | 1.660 |
| Rmerge | 0.111 | 1.117 |
| Number of reflections | 113578 | 7440 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 74.0 | |
| Redundancy | 5 | |
| CC(1/2) | 0.997 | 0.440 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | PEG 4000, AcNH4, Na citrate |
