8C6G

CRYSTAL STRUCTURE OF ODORANT BINDING PROTEIN 4 FROM ANOPHELES GAMBIAE (AGAMOBP4) AT PH 6.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-13
Detector	MAR CCD 165 mm
Wavelength(s)	1.03796
Spacegroup name	I 1 2 1
Unit cell lengths	60.355, 57.030, 67.340
Unit cell angles	90.00, 100.21, 90.00

Refinement procedure

Resolution	48.730 - 2.050
R-factor	0.195
Rwork	0.193
R-free	0.22900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3b7a
RMSD bond length	0.014
RMSD bond angle	1.441
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	2.160
High resolution limit [Å]	2.050	2.050
Rmerge	0.096	0.474
Rmeas	0.104	0.521
Rpim	0.041	0.209
Number of reflections	14115	2046
<I/σ(I)>	12.5	3.7
Completeness [%]	99.3	98.4
Redundancy	6.4	5.9
CC(1/2)	0.997	0.881

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	1.6 M TRI-SODIUM CITRATE, PH 6.5