8C68

CRYSTAL STRUCTURE OF ODORANT BINDING PROTEIN 4 FROM ANOPHELES GAMBIAE (AGAMOBP4) AT PH 4.6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I911-2
Synchrotron site	MAX II
Beamline	I911-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-13
Detector	MAR CCD 165 mm
Wavelength(s)	1.03796
Spacegroup name	P 31 2 1
Unit cell lengths	51.800, 51.800, 87.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.860 - 2.050
R-factor	0.187
Rwork	0.185
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3N88
RMSD bond length	0.011
RMSD bond angle	1.223
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.860	2.160
High resolution limit [Å]	2.050	2.050
Rmerge	0.067	0.360
Rmeas	0.071	0.384
Rpim	0.022	0.130
Number of reflections	8929	1270
<I/σ(I)>	21.1	6
Completeness [%]	99.9	99.6
Redundancy	9.5	8.4
CC(1/2)	0.999	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.6	289	2.0 M AMMONIUM SULFATE, 0.1 M SODIUM ACETATE PH 4.6