8C5H

NbSyt1 anti-(rat Synaptotagmin-1) nanobody bound to target cytosolic domain of Synaptotagmin-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-06-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	52.410, 65.750, 66.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.260 - 1.680
R-factor	0.2098
Rwork	0.209
R-free	0.22160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5t0r 6i2g
RMSD bond length	0.008
RMSD bond angle	0.953
Data reduction software	XDS
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.900	1.710
High resolution limit [Å]	1.680	1.680
Rmerge	0.087	3.822
Number of reflections	49689	1246
<I/σ(I)>	11.85	0.66
Completeness [%]	97.8	96.1
Redundancy	8.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M Di-Malic acid pH 7.0, 20% PEG 3350