8C4A

Structural and interactional insights into the glideosome-associated connector from Toxoplasma gondii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9795, 0.9796, 0.9722
Spacegroup name	P 21 21 21
Unit cell lengths	119.078, 123.605, 221.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	110.750 - 2.675
Rwork	0.209
R-free	0.26830
Structure solution method	MAD
Data reduction software	XDS (v1)
Phasing software	SHELX (v97.2)
Refinement software	REFMAC (v5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	110.750	2.771
High resolution limit [Å]	2.675	2.675
Number of reflections	904519	9144
<I/σ(I)>	14.1
Completeness [%]	99.9	99.42
Redundancy	9.9
CC(1/2)	0.993	0.681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	NA