8C3T

Structure of the GIsul2 transposon excisionase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97626
Spacegroup name	P 65
Unit cell lengths	39.305, 39.305, 71.582
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.650 - 2.110
R-factor	0.2002
Rwork	0.199
R-free	0.21400
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	0.923
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	CRANK2
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.650	19.650	2.180
High resolution limit [Å]	2.110	4.520	2.110
Number of reflections	7129	728	685
<I/σ(I)>	7.8
Completeness [%]	99.5
Redundancy	2
CC(1/2)	0.990	0.995	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	280.15	0.1M HEPES (pH 7.5) and 1.2M Lithium sulfate monohydrate