8C3I

Dark state of PAS-GAF fragment from Deinococcus radiodurans phytochrome

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	298
Detector technology	CCD
Collection date	2021-06-24
Detector	MPCCD
Wavelength(s)	1.7712
Spacegroup name	P 21 21 21
Unit cell lengths	54.950, 116.500, 117.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.070 - 2.100
R-factor	0.1626
Rwork	0.161
R-free	0.19620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6t3l
RMSD bond length	0.008
RMSD bond angle	1.084
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.070	10.300
High resolution limit [Å]	2.100	2.100
Number of reflections	50086	1821
<I/σ(I)>	10
Completeness [%]	100.0	100
Redundancy	18
CC(1/2)	0.980	0.610

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	4.95	298	60 mM Sodium Acetate, 3.3% PEG400, 1 mM DTT, 30% 2-methyl-2,4-pentanediol