8C1T

Crystal structure of Trichoplax Dlg PDZ1 domain in complex with Trichoplax Vangl peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 1
Unit cell lengths	34.696, 47.953, 50.830
Unit cell angles	85.25, 72.08, 69.17

Refinement procedure

Resolution	33.360 - 2.200
R-factor	0.2141
Rwork	0.211
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3rl7
RMSD bond length	0.010
RMSD bond angle	0.948
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.800	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	14440	1247
<I/σ(I)>	7.7
Completeness [%]	97.7
Redundancy	3.6
CC(1/2)	0.992	0.816

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	2.2 M sodium malonate dibasic monohydrate, pH 6.1 , 20% glucose