8C1N
FMDV 3D polymerase in complex with 3B1 protein solved in P212121 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 93.343, 97.024, 115.902 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.750 - 1.700 |
| R-factor | 0.2208 |
| Rwork | 0.220 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.651 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | DM |
| Refinement software | PHENIX (1.20rc4_4425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.234 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.060 | 0.858 |
| Rpim | 0.608 | |
| Number of reflections | 113370 | 14640 |
| <I/σ(I)> | 12.8 | 1.1 |
| Completeness [%] | 97.7 | 87.5 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.2M Ammonium acetate 25% PEG 4K 0.1M HEPES pH 6.5 4% gamma-butyrolactone. |
