8C10
Biochemical and structural characterisation of an alkaline family GH5 cellulase from a shipworm symbiont
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91842 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.041, 68.426, 87.188 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 53.830 - 1.000 |
| R-factor | 0.11275 |
| Rwork | 0.112 |
| R-free | 0.13342 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.256 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.040 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.077 | 0.609 |
| Number of reflections | 143241 | 5490 |
| <I/σ(I)> | 11.5 | 1.5 |
| Completeness [%] | 98.0 | 77.7 |
| Redundancy | 6.6 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.2 M MgCl2, 0.1 M Tris-HCl, pH 8.5 25 % w/v PEG 3350 |
