8C0N
Crystal structure of the red form of the mTagFT fluorescent timer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-08-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.800 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 60.645, 95.317, 95.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 95.320 - 2.900 |
| R-factor | 0.2438 |
| Rwork | 0.242 |
| R-free | 0.27730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m22 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | BUSTER |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.380 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.164 | 1.191 |
| Rmeas | 0.206 | 1.528 |
| Rpim | 0.123 | 0.945 |
| Total number of observations | 61067 | 9558 |
| Number of reflections | 23481 | 3823 |
| <I/σ(I)> | 4.7 | 1.1 |
| Completeness [%] | 97.3 | |
| Redundancy | 2.6 | 2.5 |
| CC(1/2) | 0.974 | 0.337 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 0,2 M Lithium sulfate, 0.1M Bis-tris pH 6.5, 27% PEG 3350. |
