8C0M

Crystal structure of Paradendryphiella salina PL7C alginate lyase mutant Y220F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 1
Unit cell lengths	33.973, 39.971, 42.636
Unit cell angles	74.23, 77.12, 69.81

Refinement procedure

Resolution	20.310 - 1.200
R-factor	0.1402
Rwork	0.139
R-free	0.16800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.968
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.310	1.240
High resolution limit [Å]	1.200	1.200
Number of reflections	57526	4767
<I/σ(I)>	9.31
Completeness [%]	91.3
Redundancy	3.5
CC(1/2)	0.990	0.750

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2 M Ammonium sulfate; 0.1 Sodium acetate pH 4.6