8C0A
Crystal structure of JAK2 JH2-R683S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-24 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976250 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.980, 56.695, 60.619 |
| Unit cell angles | 89.90, 79.58, 71.31 |
Refinement procedure
| Resolution | 53.610 - 1.700 |
| R-factor | 0.1962 |
| Rwork | 0.194 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fvr |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.523 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.511 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 119363 | 19114 |
| <I/σ(I)> | 7.44 | 1.07 |
| Completeness [%] | 95.0 | 94.2 |
| Redundancy | 1.82 | 1.83 |
| CC(1/2) | 0.997 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 0.1 M Tris pH 8.0, 21% PEG4000, 0.1M Na-acetate |
