8BZN

SARS-CoV-2 non-structural protein 10 (nsp10) variant T102I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-26
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9655
Spacegroup name	I 21 3
Unit cell lengths	104.867, 104.867, 104.867
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.430 - 2.190
R-factor	0.1823
Rwork	0.180
R-free	0.23530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.045
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.160	2.269
High resolution limit [Å]	2.190	2.190
Number of reflections	23664	1293
<I/σ(I)>	11.9
Completeness [%]	94.0
Redundancy	2.5
CC(1/2)	0.997	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M Bis-Tris, pH 5.5 - 6.5, 1.8 - 2.4 M NaCl